Accelerate qPCR Setup for COVID-19 Diagnosis on EzMate 601s Automated Pipetting System
Benefits
Increase speed and precision of preparing qPCR mastermix on EzMate 601s Automated Pipetting System for COVID-19 diagnostic tests
Customized method and labware layouts based on your application need
Customer's Request
During the pandemic of SARS-CoV-2, one of our customer is preparing the automation solutions for the diagnostic laboratories, including a viral RNA isolation and preparation of COVID-19 diagnostic tests based on Real-Time PCR. The company asked our help to prepare a complete methods and layout for qPCR Setup of their tests. The following are our suggestions.
Our Suggestions
1.Using EzMate 601s Automated Pipetting Sytem to prepare the Master Mix:
If the user would like to use liguid handling robot to prepare the Maser Mix (19 ul Super Mix + 1ul Enzyme Mix), the EzMate 601s Automated Pipetting System can be implemented to set up maximum 50 tests per run. (2 pieces of the Real Time RT-PCR Kit)
-Blue color: sample tubes (1.5/2ml tube)
-Purple color (1) (4): Super Mix
-Purple color (2) (5): Enzyme Mix
-Purple color (3) (6): Tube for storing Master Mix (Super Mix + Enzyme Mix)
-Red color : PCR tube (8 or 12 strip tube)
-Green color: Tips
2.Prepare the Maser Mix manually :
If the user prefers preparing the Maser Mix (19 ul Super Mix + 1ul Enzyme Mix) manually, the EzMate 601s Automated Pipetting System can set up maximum 75 tests per run (3 pieces of the Real Time RT-PCR Kit)
PCR/qPCR Set Up Related Applications
- Evaluate the Performance of Real-time PCR Instruments by Using EzMate 401 Automated Pipetting System
Learn More About EzMate Automated Pipetting System
We offer customized solutions to suit your experiment need, contact us for further discussion.